Ryan A F, Catanzaro A
Acta Otolaryngol. 1983 Jan-Feb;95(1-2):123-30. doi: 10.3109/00016488309130925.
Antigenic challenge in the middle ear of animals previously sensitized intradermally results in an immunologically mediated inflammatory response, including the generation of serous effusion in the middle ear cavity. The mechanisms which contribute to this immune-mediated otitis media with effusion (OME) were investigated by passive transfer with either purified IgG or with sensitized lymphocytes. A peritoneal exudate was raised in strain 13 guinea pigs by repeated injection of keyhole limpet hemocyanin (KLH) with complete Freund's adjuvant. IgG and lymphocytes were isolated from exudates and used for passive sensitization. Middle ear challenge following passive transfer of IgG specific to KLH produced effusion and inflammation which was equivalent to that observed in actively immunized animals, but resulted in significantly greater numbers of leukocytes infiltrating the middle ear. Unlike in the active response, few of the cells infiltrating the middle ear mucosa and middle ear cavity were eosinophils. Middle ear challenge with KLH following passive transfer with sensitized lymphocytes produced no middle ear effusion, relatively little mucosal inflammation, and sharply reduced exocytosis of leukocytes into the middle ear when compared with the active immune response or even with unimmunized controls. It is concluded that middle ear effusion, inflammation, and leukocytic exocytosis are mediated primarily by the IgG fraction of humoral immunity in our model of immune OME.
对先前经皮内致敏的动物的中耳进行抗原攻击，会引发免疫介导的炎症反应，包括中耳腔内浆液性渗出液的产生。通过用纯化的IgG或致敏淋巴细胞进行被动转移，研究了导致这种免疫介导的渗出性中耳炎（OME）的机制。通过用钥孔戚血蓝蛋白（KLH）与完全弗氏佐剂反复注射，在13号品系豚鼠中引发腹腔渗出液。从渗出液中分离出IgG和淋巴细胞，并用于被动致敏。对KLH特异性IgG进行被动转移后进行中耳攻击，会产生渗出液和炎症，这与在主动免疫动物中观察到的情况相当，但导致浸润中耳的白细胞数量显著增加。与主动反应不同，浸润中耳黏膜和中耳腔的细胞中很少有嗜酸性粒细胞。与主动免疫反应甚至未免疫的对照组相比，用致敏淋巴细胞进行被动转移后用KLH进行中耳攻击不会产生中耳渗出液，黏膜炎症相对较少，并且白细胞向中耳的胞吐作用急剧减少。得出的结论是，在我们的免疫性OME模型中，中耳渗出液、炎症和白细胞胞吐主要由体液免疫的IgG部分介导。
