Ryan A F, Cleveland P H, Hartman M T, Catanzaro A
Ann Otol Rhinol Laryngol. 1982 Jan-Feb;91(1 Pt 1):70-5. doi: 10.1177/000348948209100116.
Serum levels of specific IgG and the sensitization of peripheral blood T-lymphocytes were measured in guinea pigs after single-dose antigenic sensitization by two routes: intratympanic and intradermal injection. Keyhole limpet hemocyanin (KLH) served as the antigen. Intratympanic injection of antigen resulted in much lower levels of circulating anti-KLH IgG than intradermal injection. When KLH was conjugated with alum to produce nonspecific inflammation and serve as adjuvant, the intratympanic route was considerably enhanced, but remained much less effective than the intradermal route. Development of an IgG response was also somewhat less rapid following intratympanic than following intradermal administration. Marked sensitization of circulating T-lymphocytes was seen after intradermal injection of alum-precipitated KLH. A much weaker, though still positive, response was seen after intradermal injection of KLH alone and with the intratympanic injection of alum-precipitated KLH. No T-lymphocyte sensitization could be detected after intratympanic injection of KLH alone. It was concluded that the afferent limb of both humoral (IgG) and cell-mediated immunity was operative in the middle ear. Therefore, the middle ear does not represent an immunologically "privileged" site. On the other hand, the afferent limb from the middle ear appears to operate less effectively and rapidly than that from the dermis. This observation is consistent with observations in other mucosal systems.
通过鼓室内和皮内注射两种途径对豚鼠进行单剂量抗原致敏后，检测其血清中特异性IgG水平以及外周血T淋巴细胞的致敏情况。以钥孔戚血蓝蛋白（KLH）作为抗原。鼓室内注射抗原后，循环抗KLH IgG水平远低于皮内注射。当KLH与明矾结合以产生非特异性炎症并用作佐剂时，鼓室内途径显著增强，但仍远不如皮内途径有效。鼓室内给药后IgG反应的发展也比皮内给药稍慢。皮内注射明矾沉淀的KLH后可见循环T淋巴细胞明显致敏。单独皮内注射KLH以及鼓室内注射明矾沉淀的KLH后可见较弱但仍为阳性的反应。单独鼓室内注射KLH后未检测到T淋巴细胞致敏。得出的结论是，体液免疫（IgG）和细胞介导免疫的传入支在中耳均起作用。因此，中耳并不代表免疫“特权”部位。另一方面，来自中耳的传入支似乎比来自真皮的传入支作用更弱且更慢。这一观察结果与其他黏膜系统中的观察结果一致。
