Shain S A, Boesel R W, Klipper R W, Lancaster C M
Clin Chem. 1983 May;29(5):832-5.
We examined the effect of storing human plasma or extracts of prostate at -90 degrees C on the activity of creatine kinase and lactate dehydrogenase and isoenzyme distribution. Enzyme activities were unaltered during storage for as long as six weeks. If these preparations were thawed only once at 2 to 4 degrees C, they could be stored for as long as 165 days at -90 degrees C with no change in isoenzyme distribution. Inexplicably, apparent isoenzyme distribution of prostatic lactate dehydrogenase was sensitive to sample dilution, whereas the isoenzyme distribution of lactate dehydrogenase in plasma was not. Our observations emphasize the importance of validating details of analytical protocols that are to be used for quantification of new types of specimens.
我们研究了将人血浆或前列腺提取物在-90℃下储存对肌酸激酶和乳酸脱氢酶活性及同工酶分布的影响。在长达六周的储存期间，酶活性未发生改变。如果这些制剂仅在2至4℃下解冻一次，它们可以在-90℃下储存长达165天，同工酶分布无变化。 inexplicably，前列腺乳酸脱氢酶的表观同工酶分布对样品稀释敏感，而血浆中乳酸脱氢酶的同工酶分布则不然。我们的观察结果强调了验证用于新型标本定量分析方案细节的重要性。
