Tsukita S, Tsukita S, Ishikawa H
Cell Tissue Res. 1983;229(2):233-42. doi: 10.1007/BF00214972.
Association of actin filaments and intermediate, 10 nm filaments with the dense bodies in smooth muscle cells of the chicken gizzard was studied by thin-section and freeze-etch-replica electron microscopy. For thin-section electron microscopy we used the isolated dense bodies with attached filaments. Actin filaments appeared to be inserted into both ends (poles) of individual oblong dense bodies in such a way that arrowheads with HMM S-1 pointed away from the dense body. 10 nm filaments were attached laterally to the dense body in a side-to-side fashion. This site-specific association of actin and 10 nm filaments with the dense body was confirmed by the freeze-etch replica observations on Triton-treated smooth muscles.
利用超薄切片和冷冻蚀刻复型电子显微镜技术，研究了鸡砂囊平滑肌细胞中肌动蛋白丝及10纳米中间丝与致密体的关联。对于超薄切片电子显微镜检查，我们使用了带有附着丝的分离致密体。肌动蛋白丝似乎以这样一种方式插入单个长方形致密体的两端（极），即带有重酶解肌球蛋白S-1的箭头指向远离致密体的方向。10纳米丝以并排的方式横向附着于致密体。通过对经曲拉通处理的平滑肌进行冷冻蚀刻复型观察，证实了肌动蛋白和10纳米丝与致密体的这种位点特异性关联。
