Sato Y, Izumiya K, Sato H, Cowell J L, Manclark C R
Infect Immun. 1981 Mar;31(3):1223-31. doi: 10.1128/iai.31.3.1223-1231.1981.
Gamma globulins were obtained from rabbits hyperimmunized with the filamentous hemagglutinin (FHA) or the leukocytosis-promoting factor hemagglutinin (LPF.HA) of Bordetella pertussis. Double immunodiffusion analyses showed the antibodies to be highly specific for the homologous antigen. Intraperitoneal injection of anti-FHA or anti-LPF.HA into 10-day-old ICR mice 30 min before aerosol challenge with B. pertussis protected the mice from disease as evidenced by survival and a normal rate of gain in body weight. Both anti-LPF.HA and anti-FHA enhanced the clearance of B. pertussis from the lung and prevented leukocytosis which normally occurs after aerosol infection of mice with B. pertussis. Anti-LPF.HA, but not anti-FHA, neutralized the leukocytosis-promoting and histamine-sensitizing activities of LPF.HA when incubated with the purified protein in vitro. Anti-FHA, but not anti-LPF.HA, prevented the attachment of B. pertussis to mammalian cells (HeLa and Vero) in culture.
γ球蛋白是从用百日咳博德特氏菌的丝状血凝素（FHA）或促白细胞增多因子血凝素（LPF.HA）进行超免疫的兔子中获得的。双向免疫扩散分析表明，这些抗体对同源抗原有高度特异性。在用百日咳博德特氏菌进行气溶胶攻击前30分钟，向10日龄的ICR小鼠腹腔注射抗FHA或抗LPF.HA，可保护小鼠免受疾病侵害，这通过存活情况和正常的体重增加率得以证明。抗LPF.HA和抗FHA都增强了肺部百日咳博德特氏菌的清除，并防止了在用百日咳博德特氏菌气溶胶感染小鼠后通常会出现的白细胞增多。当在体外与纯化蛋白一起孵育时，抗LPF.HA而非抗FHA中和了LPF.HA的促白细胞增多和组胺致敏活性。抗FHA而非抗LPF.HA阻止了百日咳博德特氏菌在培养物中与哺乳动物细胞（HeLa和Vero）的附着。
Zotero 插件
