Kubota K, Chu B, Fan S F, Dewey M M, Brink P, Colflesh D E
J Mol Biol. 1983 May 25;166(3):329-40. doi: 10.1016/s0022-2836(83)80088-6.
Photoelectron count autocorrelation function of light scattered by Limulus thick myofilament suspensions was measured as a function of scattering angle in the relaxed, activated and rerelaxed states. By using the cumulants method of data analysis, the average linewidth over large ranges of KL (up to 120), has been calculated with K and L being, respectively, the magnitude of the momentum transfer vector and the length of the myofilament. We have observed a dramatic increase in the average linewidth denoting the presence of additional high frequency components for the myofilament suspension on the activated state. By confirming our results on the size of the myofilaments from electron micrographs, we are able to attribute the high frequency (kHz) components to the "correlated" cross-bridge motions, representing, to our knowledge, the first direct experimental evidence of such movements in isolated thick myofilament suspensions.
测量了鲎粗肌丝悬浮液散射光的光电子计数自相关函数，该函数是松弛、激活和再松弛状态下散射角的函数。通过使用数据分析的累积量方法，计算了KL大范围（高达120）上的平均线宽，其中K和L分别是动量传递矢量的大小和肌丝的长度。我们观察到平均线宽显著增加，这表明激活状态下肌丝悬浮液存在额外的高频成分。通过电子显微镜照片确认我们关于肌丝大小的结果，我们能够将高频（kHz）成分归因于“相关”的横桥运动，据我们所知，这是孤立粗肌丝悬浮液中此类运动的首个直接实验证据。
