Rao G A, Abraham S
Lipids. 1983 Apr;18(4):335-8. doi: 10.1007/BF02534711.
Slices, prepared from the mammary glands of lactating mice, were incubated with either [1-14C]acetate, [U-14C]glucose, or [1-14C]decanoate. From all 3 substrates, radioactivity in the synthesized lipids was found mainly in triacylglycerols (TG). When acetate or glucose served as substrate, decanoate (C10) accounted for 24% of the fatty acids in TG. Hydrolysis of the TG by pancreatic lipase yielded [14C] fatty acids which had relatively more C10 (38%) than did either of the other hydrolysis products mono- or diacylglycerol (14-17%). However, when TG produced by slices from C10 were hydrolyzed, the acid was found to be esterified equally at the C-1, C-2 and C-3 of glycerol. Thus, when fatty acids are synthesized de novo and are converted to TG by gland slices, C10 is predominantly located in the C-3 position, a finding in accord with the situation in milk TG, although such preferential incorporation does not occur when the free acid is presented to the tissue slices.
从泌乳小鼠的乳腺制备切片，将其与[1-¹⁴C]乙酸盐、[U-¹⁴C]葡萄糖或[1-¹⁴C]癸酸盐一起孵育。在所有这三种底物中，合成脂质中的放射性主要存在于三酰甘油（TG）中。当乙酸盐或葡萄糖作为底物时，癸酸盐（C10）占TG中脂肪酸的24%。用胰脂肪酶水解TG产生的[¹⁴C]脂肪酸中C10的含量（38%）比其他水解产物单酰甘油或二酰甘油（14 - 17%）相对更多。然而，当由癸酸盐产生的切片产生的TG被水解时，发现该酸在甘油的C-1、C-2和C-3位的酯化程度相同。因此，当脂肪酸从头合成并被腺体切片转化为TG时，C10主要位于C-3位，这一发现与乳TG中的情况一致，尽管当游离酸提供给组织切片时不会发生这种优先掺入。
