Hansen H O, Grunnet I, Knudsen J
Biochem J. 1984 Jun 1;220(2):521-7. doi: 10.1042/bj2200521.
ATP alone had no effect on incorporation of fatty acids synthesized de novo and membrane-bound diacylglycerol into triacylglycerol. Combined addition of ATP and Mg2+ totally inhibits incorporation of fatty acids synthesized de novo and stimulated incorporation of membrane-bound diacylglycerol. ATP, Mg2+ and glycerol 3-phosphate stimulate incorporation of fatty acids synthesized de novo into triacylglycerol, but inhibited the incorporation of membrane-bound diacylglycerol. Diacylglycerol generated in situ was shown to be superior to diacylglycerols preloaded on the membrane as substrate for the diacylglycerol acyltransferase. A model is proposed to explain the effect of absorbed exogenous fatty acid on fatty acid synthesis de novo in goat mammary gland.
单独的ATP对从头合成的脂肪酸以及膜结合二酰甘油掺入三酰甘油没有影响。ATP和Mg2+的联合添加完全抑制了从头合成的脂肪酸的掺入，并刺激了膜结合二酰甘油的掺入。ATP、Mg2+和3-磷酸甘油刺激从头合成的脂肪酸掺入三酰甘油，但抑制了膜结合二酰甘油的掺入。原位生成的二酰甘油被证明作为二酰甘油酰基转移酶的底物优于预先加载在膜上的二酰甘油。提出了一个模型来解释吸收的外源脂肪酸对山羊乳腺中从头脂肪酸合成的影响。
