Kline B J, Wood J H, Beightol L A
Arzneimittelforschung. 1983;33(4):504-6.
A high-performance liquid chromatography (HPLC) procedure has been developed for the determination of azapropazone and its 6-hydroxy metabolite in plasma and urine. The procedure is simple and rapid since extraction of the drug and its metabolite is not required. Plasma proteins are precipitated with methanol, and the supernate is injected onto a reverse-phase HPLC column. With photometric detection at 254 nm, the procedure can determine as little as 0.4 micrograms/ml azapropazone in plasma or urine and 1.5-3 micrograms/ml of the metabolite in urine, depending upon the level of interference. The procedure has been used successfully in single-dose pharmacokinetic and bioavailability studies of azapropazone dosage forms.
已开发出一种高效液相色谱（HPLC）方法，用于测定血浆和尿液中的阿扎丙宗及其6-羟基代谢物。该方法简单快速，因为无需提取药物及其代谢物。用甲醇沉淀血浆蛋白，将上清液注入反相HPLC柱。在254nm处进行光度检测，该方法可测定血浆或尿液中低至0.4微克/毫升的阿扎丙宗以及尿液中1.5 - 3微克/毫升的代谢物，具体取决于干扰水平。该方法已成功用于阿扎丙宗剂型的单剂量药代动力学和生物利用度研究。
