Action of hydrolyzed cisplatin and some analogs on microtubule protein polymerization in vitro.

The inhibition of tubulin microtubule polymerization by cisplatin was investigated. The interaction was monitored by turbidity measurements and electron microscopy. For a 2.5 x 10(-5) M concentration of cisplatin, the inhibition at 37 degrees C was nearly 50% after 1 hour of incubation and 80% after 2 hours. A similar but lesser effect was observed after incubation at 4 degrees C. One analog of cisplatin, sulfato-aqua(1,2)diamminocyclohexane platinum II, showed the same inhibition effect.