Jaffe L A, Cross N L, Picheral B
Dev Biol. 1983 Aug;98(2):319-26. doi: 10.1016/0012-1606(83)90362-7.
Fertilization of frog eggs by frog sperm is inhibited if the egg's membrane potential is positive (N.L. Cross and R.P. Elinson, 1980, Dev. Biol. 75, 187-198); however, fertilization of salamander eggs by salamander sperm does not depend on membrane potential (M. Charbonneau, M. Moreau, B. Picheral, J.P. Vilain, and P. Guerrier, 1983, Dev. Biol. 98, 304-318). Since salamander sperm can fertilize frog eggs, we have investigated whether this cross-fertilization is voltage dependent. If, during insemination with Notophthalmus sperm, Xenopus eggs were voltage clamped between +7 and +20 mV, fertilization proceeded in 7/10 (70%) of the clamped eggs, compared to 38/48 (79%) of the neighboring eggs. In control experiments in which voltage-clamped Xenopus eggs were inseminated with Xenopus sperm, fertilization proceeded in only 1/10 (10%) of the clamped eggs, compared to 59/60 (98%) of the neighbors. Similar results were obtained with cross-fertilization experiments between Notophthalmus sperm and Rana eggs. These experiments indicate that the voltage dependence of fertilization depends on the species of sperm.
如果蛙卵的膜电位为正，则蛙精子对蛙卵的受精作用会受到抑制（N.L.克罗斯和R.P.埃林森，1980年，《发育生物学》第75卷，第187 - 198页）；然而，蝾螈精子对蝾螈卵的受精作用并不依赖于膜电位（M.沙博诺、M.莫罗、B.皮切拉尔、J.P.维兰和P.盖里耶，1983年，《发育生物学》第98卷，第304 - 318页）。由于蝾螈精子能够使蛙卵受精，我们研究了这种交叉受精是否依赖于电压。在用美西螈精子授精期间，如果将非洲爪蟾卵的膜电位钳制在 +7至 +20毫伏之间，10个钳制卵中有7个（70%）发生了受精，而相邻未钳制卵中有48个中的38个（79%）发生了受精。在对照实验中，用非洲爪蟾精子对膜电位钳制的非洲爪蟾卵授精时，10个钳制卵中只有1个（10%）发生了受精，而相邻未钳制卵中有60个中的59个（98%）发生了受精。在用美西螈精子和林蛙卵进行的交叉受精实验中也得到了类似结果。这些实验表明，受精的电压依赖性取决于精子的种类。
