Doscherholmen A, Silvis S, McMahon J
Am J Clin Pathol. 1983 Oct;80(4):490-5. doi: 10.1093/ajcp/80.4.490.
A prototype food-bound vitamin B12 (food-B12) absorption test has been developed in which 57Co-B12 was incorporated in vitro into egg yolk (yolk-B12) and served to volunteers in 50-g cooked portions together with toast and coffee for breakfast. Six hours later, 1 mg nonlabeled B12 was given intramuscularly and 24-hour urine was collected for radioactivity measurement. In separate tests, the absorption of yolk-B12 and crystalline 57Co-B12 was equally poor in patients with pernicious anemia. However, in patients with simple gastric achlorhydria and those who had undergone gastric surgery, the assimilation of yolk-B12 was impaired greatly, whereas the absorption of crystalline radio-B12 was normal. Egg yolk labeled with 58Co-B12 was administered together with crystalline 57Co-B12 in a dual isotope test with results similar to those obtained when the tests were prepared separately. This yolk-58Co-B12 test with its ability to detect malabsorption of food-B12 may be considered as an addition to the first part of the Schilling test.
已开发出一种原型食物结合型维生素B12（食物B12）吸收试验，其中57Co-B12在体外被掺入蛋黄中（蛋黄B12），并以50克煮熟的量与烤面包和咖啡一起作为早餐提供给志愿者。6小时后，肌肉注射1毫克非标记B12，并收集24小时尿液进行放射性测量。在单独的试验中，恶性贫血患者对蛋黄B12和结晶57Co-B12的吸收同样很差。然而，在单纯性胃酸缺乏患者和接受过胃手术的患者中，蛋黄B12的同化作用大大受损，而结晶放射性B12的吸收正常。在双同位素试验中，将用58Co-B12标记的蛋黄与结晶57Co-B12一起给药，结果与单独进行试验时相似。这种蛋黄58Co-B12试验能够检测食物B12吸收不良，可被视为希林试验第一部分的补充。
