一种适用于人类的哺乳动物肾25-羟维生素D3-1-羟化酶的微量测定法。

A microassay for mammalian renal 25-hydroxyvitamin D3-1-hydroxylase applicable to man.

作者信息

Tanaka Y, DeLuca H F, Satomura K, Yamaoka K, Seino Y

出版信息

J Lab Clin Med. 1983 Dec;102(6):1010-6.

Abstract

A micro determination of the renal 25-hydroxyvitamin D3-1-hydroxylase activity was developed that uses as little as 1 mg of rat kidney tissue. The method was applied to the remaining portions of needle kidney biopsy specimens taken for diagnostic purposes from children who had asymptomatic proteinuria and/or hematuria (more than 1-year duration) but were otherwise normal in calcium metabolism and renal function. The 25-hydroxyvitamin D3-1-hydroxylase levels of these children were found to be 66 1,25-dihydroxyvitamin D3 per milligram of tissue per 20 min for an 11-year-old male, 117 pg for a 9-year-old male, and 89 pg for a 10-year-old female.

摘要

开发了一种微量测定肾25-羟维生素D3-1-羟化酶活性的方法，该方法仅需使用1毫克大鼠肾脏组织。该方法应用于因诊断目的而取自无症状蛋白尿和/或血尿（持续时间超过1年）但钙代谢和肾功能正常的儿童的经皮肾活检标本的剩余部分。发现这些儿童的25-羟维生素D3-1-羟化酶水平分别为：一名11岁男性每毫克组织每20分钟产生66 pg 1,25-二羟维生素D3，一名9岁男性为117 pg，一名10岁女性为89 pg。