Lasky L C, Ascensao J, McCullough J, Zanjani E D
Br J Haematol. 1983 Dec;55(4):615-22. doi: 10.1111/j.1365-2141.1983.tb02843.x.
The number of committed granulocyte-monocyte precursors (CFU-GM) in circulation has been shown to increase following steroid administration in humans. To see if steroids could be used to improve the collection of peripheral blood stem cells for haematopoietic reconstitution, their effect on circulating CFU-GEMM was investigated. Six healthy young adults, three men and three women, were given 60 mg of prednisone orally at 8 a.m. on day 1. Blood CFU-GEMM and committed precursors (CFU-GM and BFU-E) were cultured in methylcellulose. Samples were taken at 4 p.m. the day before and at 8 a.m. on the day of steroid administration, and at 4, 8, 24 and, in a few cases, 48 h after steroid. Pre-treatment CFU-GEMM levels (per unit volume of blood) at 4 p.m. was 180 +/- 23% (mean +/- SEM) of that at 8 a.m., showing a significant (P less than 0.025) diurnal variation. 8 h after steroids there was a fall in CFU-GEMM to 28 +/- 4.5% of the 8 a.m. presteroid level. 24 h following steroid administration, CFU-GEMM rose significantly (P less than 0.05) to 188 +/- 33% of 8 a.m. baseline values; CFU-GM and BFU-E changes generally paralleled those noted for CFU-GEMM. These results suggest that blood levels of CFU-GEMM exhibit a significant diurnal variation. Oral prednisone given 24 h in advance of collection increases the 8 a.m. value to that found at 4 p.m. The steroid effect may be due to a resetting of the diurnal control mechanism. Use of this information may be important in collection of circulating haematopoietic stem cells for use in bone marrow reconstitution in man.
在人类中，已证实给予类固醇后循环中定向粒细胞-单核细胞前体细胞（CFU-GM）的数量会增加。为了探究类固醇是否可用于改善外周血干细胞的采集以进行造血重建，研究了其对循环中CFU-GEMM的影响。6名健康的年轻成年人，3名男性和3名女性，在第1天上午8点口服60毫克泼尼松。血液CFU-GEMM和定向前体细胞（CFU-GM和BFU-E）在甲基纤维素中培养。在给予类固醇前一天下午4点、给予类固醇当天上午8点以及给予类固醇后4、8、24小时（少数情况为48小时）采集样本。下午4点的预处理CFU-GEMM水平（每单位体积血液）为上午8点的180±23%（平均值±标准误），显示出显著的（P＜0.025）昼夜变化。给予类固醇8小时后，CFU-GEMM降至上午8点类固醇给药前水平的28±4.5%。给予类固醇24小时后，CFU-GEMM显著升高（P＜0.05）至上午8点基线值的188±33%；CFU-GM和BFU-E的变化通常与CFU-GEMM的变化相似。这些结果表明CFU-GEMM的血液水平呈现显著的昼夜变化。提前24小时口服泼尼松可使上午8点的值升高至下午4点时的值。类固醇的作用可能是由于昼夜控制机制的重新设定。利用这一信息对于采集循环造血干细胞用于人类骨髓重建可能很重要。
