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Transcription of bacteriophage T4 DNA in vitro: selective initiation with dinucleotides.

作者信息

Rüger W

出版信息

Eur J Biochem. 1978 Jul 17;88(1):109-17. doi: 10.1111/j.1432-1033.1978.tb12427.x.

DOI:10.1111/j.1432-1033.1978.tb12427.x
PMID:668702
Abstract

The transcription products of phage T4 DNA in vitro are separated on polyacrylamide gels. The influence of salt, polymerase, triphosphate concentration and glucosylation on the RNA synthesis are shown. Individual transcripts are initiated selectively with dinucleotides and a single triphosphate. This technique allows the prediction of the initiation sequences of several T4 transcripts.

摘要

相似文献

1
Transcription of bacteriophage T4 DNA in vitro: selective initiation with dinucleotides.
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IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.从两个不同启动子对噬菌体T4 tRNA基因簇进行体外转录。
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Mapping of in vitro transcription units and identification of primary transcripts of the D region of bacteriophage T4.
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