Perry H M, Chappel J C, Clevinger B L, Haddad J G, Teitelbaum S L
Biochem Biophys Res Commun. 1983 Apr 29;112(2):431-6. doi: 10.1016/0006-291x(83)91482-1.
We have developed a monoclonal antibody capable of detecting 1 pg/ml of 1,25-dihydroxycholecalciferol. At a dilution of 1:80,000 of ascitic fluid this antibody has an apparent KD of 3.3 X 10(-11) ML-1. The immunogen used was a vitamin D analogue, calcitroic acid [1 alpha, 3 beta-dihydroxy-9, 10 seco-24-nor 5, 7, 10 (19) cholatriene-23-oic acid], conjugated to bovine serum albumin. Although this antibody is extremely sensitive, it also recognizes other important vitamin D3 metabolites.
我们已经研制出一种单克隆抗体，它能够检测出1皮克/毫升的1,25 - 二羟胆钙化醇。在腹水稀释1:80,000的情况下，这种抗体的表观解离常数（KD）为3.3×10⁻¹¹摩尔⁻¹升。所使用的免疫原是一种维生素D类似物，即钙三醇酸[1α, 3β - 二羟基 - 9, 10 - 开环 - 24 - 降 - 5, 7, 10(19) - 胆三烯 - 23 - 酸]，它与牛血清白蛋白偶联。尽管这种抗体极其灵敏，但它也能识别其他重要的维生素D3代谢产物。
