Linders P W, Stols A L, Stadhouders A M
J Microsc. 1983 Apr;130(Pt 1):85-92. doi: 10.1111/j.1365-2818.1983.tb04201.x.
An improved calibration procedure is described for dry mass determination methods, that are based on microdensitometry of transmission electron micrographs. Polystyrene latex spheres, that are supposed to have a homogeneous mass distribution, are used for the calibration procedure. By computer fitting of the theoretically expected mass distribution to the distribution measured in a line through the centre of a sphere, a mass calibration factor is obtained. The number of measurements required for a predetermined calibration accuracy can be decreased considerably as compared to other calibration methods. Accurate calibration and reproducible adjustment of the electron optical magnification are required.
本文描述了一种改进的校准程序，用于基于透射电子显微镜图像微密度测定的干质量测定方法。校准程序使用了假定具有均匀质量分布的聚苯乙烯乳胶球。通过将理论预期的质量分布与通过球体中心的一条线上测量的分布进行计算机拟合，可获得质量校准因子。与其他校准方法相比，达到预定校准精度所需的测量次数可大幅减少。需要进行精确校准和可重复调整电子光学放大倍数。
