Gluzman B E, Niepomniszcze H
Acta Endocrinol (Copenh). 1983 May;103(1):34-9. doi: 10.1530/acta.0.1030034.
Kinetics of the iodide trapping mechanism in thyroid slices was studied in human and animal tissues. Slices were incubated with several medium iodide concentrations, ranging from 5 x 10(-6)M to 2 x 10(-4)M, in order to calculate in the steady state the following kinetic parameters of the iodide transport: Km, maximal capacity (C) and diffusion factor (D). Results indicated that the Km was similar in magnitude (10(-5)M) in all cases where trapping activity was present, while maximal capacity (C) values showed significant differences between those pathologies in which trapping activity was hyperstimulated (dishormonogenetic goitre, Graves' disease, toxic adenoma) and those where thyroid tissues presented focal or total alterations on its structure (non-toxic nodular goitre, Hashimoto's thyroiditis, thyroid cancer) or where thyroid tissues were not sufficiently stimulated by TSH (extranodular tissue of toxic adenoma). 'Warm' nodules were not significantly different from normal human thyroid. These results suggest that the scattered trapping values observed in the different thyroid pathologies correspond to quantitative differences between them rather than to qualitative alterations in the thyroid iodide pump.
在人体和动物组织中研究了甲状腺切片中碘捕获机制的动力学。将切片与几种碘浓度范围从5×10⁻⁶M至2×10⁻⁴M的培养基一起孵育，以便在稳态下计算碘转运的以下动力学参数：米氏常数（Km）、最大容量（C）和扩散因子（D）。结果表明，在所有存在捕获活性的情况下，Km的大小相似（10⁻⁵M），而最大容量（C）值在捕获活性被过度刺激的那些病理情况（致甲状腺肿性甲状腺肿、格雷夫斯病、毒性腺瘤）与甲状腺组织在结构上呈现局灶性或整体性改变的那些情况（非毒性结节性甲状腺肿、桥本甲状腺炎、甲状腺癌）或甲状腺组织未被促甲状腺激素充分刺激的情况（毒性腺瘤的结节外组织）之间存在显著差异。“热”结节与正常人体甲状腺无显著差异。这些结果表明，在不同甲状腺病理情况中观察到的分散的捕获值对应于它们之间的数量差异，而不是甲状腺碘泵的质量改变。
