Goran D A, Shields H M, Bates M L, Zuckerman G R, DeSchryver-Kecskemeti K
Gastroenterology. 1984 Jan;86(1):39-50.
This study was performed to determine the characteristics of esophageal dysplasia by scanning electron microscopy. A total of 82 esophageal biopsy specimens were taken from 30 patients who were divided into three groups. Group 1 patients had no known esophageal disease. Group 2 patients had squamous cell cancer. Group 3 patients had esophagitis. Mucosal biopsy specimens that had been diagnosed by light microscopy as normal, esophagitis, or dysplastic mucosa were examined by scanning electron microscopy. A characteristic appearance for each type of mucosa was recognized by scanning electron microscopy. A quantitative analysis of the scanning electron microscopy feature of microridge density showed a statistically significant difference not only between normal and dysplastic mucosa, but also between esophagitis and dysplastic mucosa. The addition of scanning electron microscopy to light microscopy may prove helpful in the diagnosis of dysplasia as well as in the understanding of the biologic behavior of dysplastic cells and possibly their relationship to esophageal carcinoma.
本研究旨在通过扫描电子显微镜确定食管发育异常的特征。从30名患者身上共采集了82份食管活检标本，这些患者被分为三组。第1组患者无已知食管疾病。第2组患者患有鳞状细胞癌。第3组患者患有食管炎。对经光学显微镜诊断为正常、食管炎或发育异常黏膜的黏膜活检标本进行扫描电子显微镜检查。通过扫描电子显微镜可识别每种黏膜类型的特征性外观。对微嵴密度的扫描电子显微镜特征进行定量分析显示，不仅正常黏膜与发育异常黏膜之间存在统计学上的显著差异，食管炎与发育异常黏膜之间也存在显著差异。在光学显微镜检查中加入扫描电子显微镜检查可能有助于发育异常的诊断，以及理解发育异常细胞的生物学行为及其与食管癌可能的关系。
