Park H, Zeiger A R, Schumacher H R
Infect Immun. 1984 Jan;43(1):139-42. doi: 10.1128/iai.43.1.139-142.1984.
A unique enzyme-linked immunosorbent assay was developed to detect soluble peptidoglycans in biological fluids. It makes use of the similar affinities of vancomycin and purified rabbit antibodies to peptidoglycan precursor sequences found in soluble peptidoglycans. This assay has been used to detect as little as 500 pg of soluble peptidoglycan per ml of serum and 5 pg/ml of urine. Studies of normal individuals and Staphylococcus aureus-infected patients revealed only a few sera with detectable levels of soluble peptidoglycans. Studies of normal volunteers who were given a single oral dose of 250 mg of penicillin VK showed that about half had detectable levels of soluble peptidoglycans in their urine up to 6 h after ingestion. This suggests that soluble peptidoglycans can be released by indigenous bacteria in detectable amounts. In one volunteer, a detectable level of soluble peptidoglycan in the urine at 6 h decreased to an undetectable level at 12 h. Such an ephemeral appearance of soluble peptidoglycan in the urine could account for the small number of human sera that had detectable levels of soluble peptidoglycan.
一种独特的酶联免疫吸附测定法被开发出来用于检测生物体液中的可溶性肽聚糖。它利用了万古霉素和纯化的兔抗体对可溶性肽聚糖中发现的肽聚糖前体序列的相似亲和力。该测定法已用于检测每毫升血清中低至500皮克的可溶性肽聚糖以及每毫升尿液中5皮克的可溶性肽聚糖。对正常个体和金黄色葡萄球菌感染患者的研究表明，只有少数血清中可检测到可溶性肽聚糖水平。对单次口服250毫克青霉素V钾的正常志愿者的研究表明，约一半人在摄入后6小时内尿液中可检测到可溶性肽聚糖水平。这表明可溶性肽聚糖可由本地细菌以可检测的量释放出来。在一名志愿者中，尿液中6小时时可检测到的可溶性肽聚糖水平在12小时时降至不可检测水平。尿液中可溶性肽聚糖的这种短暂出现可能解释了少数可检测到可溶性肽聚糖水平的人血清的情况。
