Tomana M, Prchal J T, Garner L C, Skalka H W, Barker S A
J Lab Clin Med. 1984 Jan;103(1):137-42.
Gas-liquid chromatography was employed to determine free monosaccharides in human, rat, and South American rodent Octodon degu lenses. In addition to D-glucose, D-fructose, sorbitol and myo-inositol, which were previously known to be components of lens tissue, glycerol, erythritol, threitol, ribitol, xylitol, and mannitol were also detected in the lenses of these three species. The concentrations in lenses of these three species were in the following ranges: glycerol 1.2 to 40 mumol/gm wet tissue; myo-inositol 4 to 25; and glucose, fructose, and remaining alditols 0.01 to 1.4/mumol/gm. The method is described and its applicability to analyses of free monosaccharides in other animal tissues is discussed.
采用气液色谱法测定人、大鼠以及南美啮齿动物八齿鼠晶状体中的游离单糖。除了先前已知的晶状体组织成分D-葡萄糖、D-果糖、山梨醇和肌醇外，在这三种动物的晶状体中还检测到了甘油、赤藓醇、苏糖醇、核糖醇、木糖醇和甘露醇。这三种动物晶状体中的浓度范围如下：甘油1.2至40微摩尔/克湿组织；肌醇4至25；葡萄糖、果糖和其余糖醇0.01至1.4微摩尔/克。本文描述了该方法，并讨论了其在分析其他动物组织中游离单糖方面的适用性。
