Schleef R R, Birdwell C R
Arteriosclerosis. 1984 Jan-Feb;4(1):14-20. doi: 10.1161/01.atv.4.1.14.
Endothelial cells in vivo come into contact with fibrin during numerous physiological and pathological processes, but the nature of this interaction is poorly understood. The interaction of fibrin and endothelial cells was examined by incubating bovine aortic endothelial cell monolayers in vitro with fibrinogen (100 micrograms/ml) in medium supplemented with 10% calf serum. This treatment produced a layer of fibrin above the endothelial cell monolayer, which induced disorganization of the monolayer and remained associated with the monolayer even after washing. Endothelial cell pinocytosis of 14C-sucrose was elevated by 25% and 60% after incubation with fibrin for 8 hours and 2 days, respectively. Fibrin deposition on endothelial cell monolayers increased the rate of DNA synthesis (3H-thymidine incorporation) for 1 to 3 days by as much as 60% over controls. Concomitant with the lysis of fibrin above endothelial cells and the reorganization of the monolayer at Days 4 or 5 was a reduction of endothelial cell pinocytosis and DNA synthesis to control values. In contrast, there was no effect on the cell density at any time after incubation with fibrin. These results indicate that fibrin interacts with endothelial cells and induces a disorganization of the monolayer that is accompanied by changes in the rate of pinocytosis and in the rate of DNA synthesis.
在许多生理和病理过程中，体内的内皮细胞会与纤维蛋白接触，但这种相互作用的本质却知之甚少。通过在补充有10%小牛血清的培养基中，将牛主动脉内皮细胞单层与纤维蛋白原（100微克/毫升）在体外孵育，来研究纤维蛋白与内皮细胞的相互作用。这种处理在内皮细胞单层上方产生了一层纤维蛋白，它导致了单层细胞的紊乱，并且即使在洗涤后仍与单层细胞相关联。与纤维蛋白孵育8小时和2天后，内皮细胞对14C-蔗糖的胞饮作用分别提高了25%和60%。纤维蛋白在内皮细胞单层上的沉积使DNA合成速率（3H-胸腺嘧啶掺入）在1至3天内比对照组提高了多达60%。在第4天或第5天，随着内皮细胞上方纤维蛋白的溶解和单层细胞的重新组织，内皮细胞的胞饮作用和DNA合成减少至对照值。相比之下，与纤维蛋白孵育后的任何时候，细胞密度都没有受到影响。这些结果表明，纤维蛋白与内皮细胞相互作用，并诱导单层细胞紊乱，同时伴随着胞饮作用速率和DNA合成速率的变化。
