Schleef R R, Birdwell C R
Tissue Cell. 1982;14(4):629-36. doi: 10.1016/0040-8166(82)90053-2.
Endothelial cells are known to migrate and come into contact with fibrin during numerous physiological processes, such as in wound healing and in tumor growth. The present study was initiated to investigate the effect of fibrin on endothelial cell migration in vitro. Endothelial cell migration was assayed by wounding confluent monolayers of bovine aortic endothelial cells with a razor blade and counting the number of cells crossing the wound per unit time. Wound-induced proliferation of endothelial cells was inhibited by mitomycin C-treatment without affecting endothelial cell migration, indicating that in this assay migration could be measured independent of proliferation. Migration of endothelial cells in vitro was inhibited by fibrin in a concentration dependent manner. Endothelial cell migration under fibrin was further reduced by plasminogen depletion of the serum, and fibrin still inhibited the migration of mitomycin C-treated endothelial cells. Kadish et al. (Tissue and Cell, 11, 99, 1979) previously reported that fibrin did not affect EC migration in vitro. The inability to inhibit EC migration with fibrin appears to be due to their assay system which employed agarose, since pre-treating the wounded monolayer with agarose eliminated the inhibition of EC migration by fibrin. The present results indicate that EC migration in vitro can be used as a model system for studying the interaction of fibrin with EC.
众所周知，在内皮细胞迁移并与纤维蛋白接触的众多生理过程中，比如在伤口愈合和肿瘤生长过程中均会发生这种情况。开展本研究旨在调查纤维蛋白对体外内皮细胞迁移的影响。通过用剃须刀片划伤汇合的牛主动脉内皮细胞单层，并计算单位时间内穿过伤口的细胞数量来检测内皮细胞迁移。丝裂霉素C处理可抑制伤口诱导的内皮细胞增殖，而不影响内皮细胞迁移，这表明在该检测中，迁移可独立于增殖进行测量。体外培养时，纤维蛋白以浓度依赖的方式抑制内皮细胞迁移。血清纤维蛋白原耗竭可进一步降低纤维蛋白作用下的内皮细胞迁移，并且纤维蛋白仍能抑制经丝裂霉素C处理的内皮细胞迁移。卡迪什等人（《组织与细胞》，第11卷，第99页，1979年）此前报道，纤维蛋白不影响体外内皮细胞迁移。无法用纤维蛋白抑制内皮细胞迁移似乎是由于他们使用琼脂糖的检测系统，因为用琼脂糖预处理受伤的单层细胞可消除纤维蛋白对内皮细胞迁移的抑制作用。目前的结果表明，体外内皮细胞迁移可作为研究纤维蛋白与内皮细胞相互作用的模型系统。
