Kadish J L, Butterfield C E, Folkman J
Tissue Cell. 1979;11(1):99-108. doi: 10.1016/0040-8166(79)90010-7.
The normal cobblestone monolayer architecture of cultured vascular endothelium becomes rapidly disorganized after contact of the cell layer with a fibrin clot. The cells of a confluent endothelial monolayer separate into individual migratory cells in 4--6 hr after contact with fibrin. The effect is reversible in that removal of the fibrin clot results in resumption of the normal morphology within about 2 hr. No other cell type tested exhibits the same change in organization when exposed to fibrin. A similar morphological change in endothelium does occur after the cell layer is overlaid with a collagen fibril gel but a gel of methylcellulose has no effect. It is proposed that the change in behavior of endothelial cells in response to contact with fibrin may represent a cellular component of fibrinolysis. The implications of this finding for the pathophysiology of disease states involving intravascular fibrin deposition are discussed.
培养的血管内皮细胞正常的鹅卵石状单层结构在细胞层与纤维蛋白凝块接触后会迅速紊乱。融合的内皮单层细胞在与纤维蛋白接触后4 - 6小时会分离成单个迁移细胞。这种效应是可逆的，因为去除纤维蛋白凝块后，大约2小时内细胞会恢复正常形态。当暴露于纤维蛋白时，测试的其他细胞类型均未表现出相同的组织变化。当细胞层覆盖有胶原纤维凝胶时，内皮细胞也会发生类似的形态变化，但甲基纤维素凝胶则没有影响。有人提出，内皮细胞与纤维蛋白接触后行为的改变可能代表纤维蛋白溶解的一个细胞成分。本文讨论了这一发现对涉及血管内纤维蛋白沉积的疾病状态病理生理学的影响。
