Iudina I E, Lemeneva Z L, Okulov A B, Fedorova K N
Biull Eksp Biol Med. 1984 Jan;97(1):81-3.
Interphase chromatin of peripheral lymphocytes was studied in patients aged 6 to 20 years with Turner and Morris's syndromes by AO labeled fluorometry using the authors' modification of DNP cell thermal denaturation. It was shown that the lymphocyte chromatin melting profiles represent the curves with seven maxima at the following temperatures: 47 degrees, 55 degrees, 65 (+/- 2)degrees, 78 (+/- 1)degrees, 82, 88 (+/- 1)degrees, 92 (+/- 2)degrees C (P less than 0.01). There were no statistically significant differences between clinically and karyotypically different groups either in the parallelism of the melting profiles or in the fluorescence intensity of AO connected with cell DNP. In the male control group, the similar curve was obtained for lymphocyte chromatin in 25 and for normal human spermatozoa in 100% of cases, i.e. deviation specificity was revealed in the lymphocyte melting profiles, from a "classical" normal variant to the so-called male variant with sex differentiation breaks (Turner, Morris and Klinefelter's syndromes). Possible cytogenetic mechanisms of breaks are discussed.
利用作者改进的二硝基苯酚（DNP）细胞热变性法，通过吖啶橙（AO）标记荧光法对6至20岁患有特纳综合征和莫里斯综合征的患者外周淋巴细胞的间期染色质进行了研究。结果表明，淋巴细胞染色质的解链曲线在以下温度下出现七个峰值：47℃、55℃、65（±2）℃、78（±1）℃、82℃、88（±1）℃、92（±2）℃（P<0.01）。临床和核型不同的组之间，无论是解链曲线的平行度还是与细胞DNP相关的AO荧光强度，均无统计学显著差异。在男性对照组中，25例淋巴细胞染色质以及100%的正常人精子均获得了相似的曲线，即淋巴细胞解链曲线显示出特异性偏差，从“经典”正常变体到具有性分化中断的所谓男性变体（特纳综合征、莫里斯综合征和克氏综合征）。文中讨论了断裂可能的细胞遗传学机制。
