Iudina I E, Fedorova K N
Biull Eksp Biol Med. 1984 Nov;98(11):589-91.
It was shown with labeled AO cytofluorometry using the authors' modification of DNP cell thermal denaturation that in untreated patients with chronic myeloid leukemia (CML) in the blast crisis phase, the melting profiles of peripheral blood cell chromatin were "unclassified", strictly individual in nature (unlike the identical parameter in healthy subjects) that may attest to regulatory disturbances of the genetic apparatus. The demonstration of the "normalization" of the melting profiles of chromatin in CML patients during the clinico-hematological compensation of the disease makes it possible to suggest that luminescent fluorometry (in the authors' modification of DNP cell thermal denaturation) should be used for the treatment of hemoblastoses in order to study the genesis and time-course of the diseases running their course with remissions and as criterion of drug therapy of these diseases.
采用作者改良的二硝基苯酚（DNP）细胞热变性标记AO细胞荧光测定法表明，在未经治疗处于原始细胞危象期的慢性髓性白血病（CML）患者中，外周血细胞染色质的解链曲线是“未分类的”，本质上是严格个体化的（与健康受试者的相同参数不同），这可能证明遗传装置存在调节紊乱。CML患者在疾病临床血液学缓解期染色质解链曲线“正常化”的证明表明，发光荧光测定法（作者改良的DNP细胞热变性法）应用于血液系统恶性肿瘤的治疗，以研究疾病的发生和病程以及作为这些疾病药物治疗的标准。
