Yunis J J
Cancer Genet Cytogenet. 1984 Feb;11(2):125-37. doi: 10.1016/0165-4608(84)90106-7.
Using a methotrexate cell synchronization technique, we have successfully studied chromosome preparations of bone marrow biopsies from 49 of 51 patients with acute nonlymphocytic leukemia (ANLL). Clonal chromosomal abnormalities were detected in 46 patients, and one of eight types of recurrent chromosomal aberrations were found in 31 patients. One of these types represents a new chromosome defect [inv(16)(p13.1q22)], found in four patients with ANLL-M2, -M4. Chromosome preparations from cultured lymphocytes of two of the four patients were analyzed for the presence of chromosome fragile sites. Both cases showed a frequent break at band 16q22, an intriguing finding that suggests a possible correlation between a fragile site and predisposition to chromosomal rearrangement in human neoplasia.
采用甲氨蝶呤细胞同步化技术，我们成功地对51例急性非淋巴细胞白血病（ANLL）患者中的49例进行了骨髓活检的染色体标本研究。在46例患者中检测到克隆性染色体异常，31例患者中发现了八种复发性染色体畸变类型中的一种。其中一种类型代表一种新的染色体缺陷[inv(16)(p13.1q22)]，在4例ANLL-M2、-M4患者中发现。对这4例患者中的2例培养淋巴细胞的染色体标本进行了分析，以检测染色体脆性位点的存在。两例均显示16q22带频繁断裂，这一有趣的发现提示脆性位点与人类肿瘤中染色体重排易感性之间可能存在关联。
