Li A K, Koroly M J, Schattenkerk M E, Malt R A, Young M
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4379-81. doi: 10.1073/pnas.77.7.4379.
Earlier studies have shown that removal of the submandibular glands of mice retards the rate of contraction of experimentally induced wounds and that communal licking of wounds accelerates contraction in intact animals [Hutson, J. M., Niall, M., Evans, D. & Fowler, R. (1979) Nature (London) 279, 793-795]. In the light of the observation that nerve growth factor (NGF) is secreted in high concentrations in mouse saliva, we have studied the effect of topically applied high molecular weight nerve growth factor (HMW-NGF) upon the rate of wound contraction in sialoadenectomized animals. Results show that HMW-NGF significantly accelerates the rate of wound contraction and that this phenomenon is probably dependent upon the enzymic activity of the protein. Neither diisopropyl fluorophosphate-inactivated NGF nor 2.5S NGF [isolated according to Bocchini, V. & Angeletti, P. U. (1969) Proc. Natl. Acad. Sci. USA 64, 787-794] displays this biological activity. Thus, it may be that one of the physiological roles of NGF in saliva is to promote wound healing by the licking process.
早期研究表明，切除小鼠的下颌下腺会延缓实验性诱导伤口的收缩速度，而在完整动物中，共同舔舐伤口会加速收缩[赫特森，J.M.，尼尔，M.，埃文斯，D.和福勒，R.（1979年）《自然》（伦敦）279，793 - 795]。鉴于观察到神经生长因子（NGF）在小鼠唾液中高浓度分泌，我们研究了局部应用高分子量神经生长因子（HMW - NGF）对唾液腺切除动物伤口收缩速度的影响。结果表明，HMW - NGF显著加速了伤口收缩速度，并且这种现象可能取决于该蛋白质的酶活性。二异丙基氟磷酸灭活的NGF和2.5S NGF[根据博基尼，V.和安杰莱蒂，P.U.（1969年）《美国国家科学院院刊》64，787 - 794分离]均未表现出这种生物活性。因此，NGF在唾液中的生理作用之一可能是通过舔舐过程促进伤口愈合。
