Meyer G, Kloppstech K
Eur J Biochem. 1984 Jan 2;138(1):201-7. doi: 10.1111/j.1432-1033.1984.tb07900.x.
Among the translation products obtained in vitro with mRNAs isolated from etiolated grown pea, or after different times of illumination following the etiolation, a 24000-Mr protein has been observed in the very early phase of greening; its occurrence culminates at 2-4 h after the start of illumination. From these data it is concluded that the corresponding mRNA appears in and disappears from the poly(A)-containing RNA population within hours. The protein product has been characterized as the precursor for a 17000-Mr chloroplast protein; by means of post-translational transport in vitro, the processed product becomes bound to chloroplast membranes. A product of the same size can also be labeled in vivo with a maximum of incorporation of label at 6-8 h after illumination. This product decays with a half-life of about 5 h. These findings imply a regulatory function of the 17000-Mr protein during the process of greening, possibly by synchronization of nuclear and chloroplast genomes. Other possibilities are considered.
在用从黄化生长的豌豆中分离的mRNA体外翻译得到的产物中，或者在黄化后的不同光照时间后，在绿化的早期阶段观察到一种分子量为24000的蛋白质；其出现量在光照开始后2-4小时达到峰值。从这些数据可以得出结论，相应的mRNA在数小时内出现在含聚腺苷酸的RNA群体中并消失。该蛋白质产物已被鉴定为一种分子量为17000的叶绿体蛋白质的前体；通过体外翻译后转运，加工后的产物与叶绿体膜结合。同样大小的产物在体内也可以被标记，光照后6-8小时标记掺入量最大。该产物以约5小时的半衰期衰变。这些发现意味着分子量为17000的蛋白质在绿化过程中具有调节功能，可能是通过同步核基因组和叶绿体基因组来实现的。还考虑了其他可能性。
