Grimm B, Kloppstech K
Institut für Botanik, Universität Hannover, Federal Republic of Germany.
Eur J Biochem. 1987 Sep 15;167(3):493-9. doi: 10.1111/j.1432-1033.1987.tb13364.x.
Within 1-2 h of illumination of etiolated barley plants the mRNAs of seven nuclear-coded proteins are transiently induced. It is proposed that at least some of these proteins are precursors to chloroplast membrane proteins since after posttranslational transport 2-h-specific bands of 18.5 kDa, 18 kDa and 13.5 kDa have been found bound to thylakoid membranes. cDNA clones for these early light-inducible proteins (ELIPs) have been isolated. Hybrid-release translation shows that part of their information must be homologous since the complete set of early light-inducible translation products is obtained with all investigated clones although the proportions of the translated bands vary for individual clones. From hybridization data it is concluded that two ELIP families of high (24-27 kDa) and of low (16-18 kDa) molecular mass exist which are induced in parallel. Induction of ELIPs occurs even at very low light intensities and is saturated at about 1000 lx. Therefore, ELIPs are not considered to represent light stress proteins but to play a regulatory role during development.
在黄化大麦植株光照1 - 2小时内，七种核编码蛋白的mRNA被短暂诱导。有人提出，这些蛋白中至少有一些是叶绿体膜蛋白的前体，因为在翻译后转运后，已发现18.5 kDa、18 kDa和13.5 kDa的2小时特异性条带与类囊体膜结合。这些早期光诱导蛋白（ELIPs）的cDNA克隆已被分离出来。杂交释放翻译表明，它们的部分信息一定是同源的，因为尽管各个克隆翻译条带的比例不同，但用所有研究的克隆都能获得完整的早期光诱导翻译产物集。从杂交数据得出结论，存在两个高分子量（24 - 27 kDa）和低分子量（16 - 18 kDa）的ELIP家族，它们是平行诱导的。即使在非常低的光照强度下也会发生ELIPs的诱导，并且在约1000勒克斯时达到饱和。因此，ELIPs不被认为是光胁迫蛋白，而是在发育过程中起调节作用。
