Francavilla A, di Leo A, Eagon P K, Wu S Q, Ove P, van Thiel D H, Starzl T E
Gastroenterology. 1984 Mar;86(3):552-7.
Estrogen receptor activity was quantitated in the cytosol and nucleus of normal rat liver and in regenerating rat liver at several time intervals after 75% hepatectomy. Cytosolic estradiol binding in regenerating liver decreases at 12, 24, and 48 h after hepatectomy and at 48 h is 30% of that in normal rat liver. Nuclear estrogen binding 48 h after surgery is elevated fivefold over normal values. No alterations in affinity of the receptor for estrogen have been observed. Specificity studies indicate that the estrogen receptors from both normal and regenerating liver were similar and are highly specific for estrogens. These changes in cellular distribution of receptors parallel increases in nuclear deoxyribonucleic acid synthesis and mitotic indices in the liver.
在75%肝切除术后的几个时间点，对正常大鼠肝脏以及再生大鼠肝脏的细胞质和细胞核中的雌激素受体活性进行了定量分析。肝切除术后12、24和48小时，再生肝脏中的细胞质雌二醇结合量减少，48小时时仅为正常大鼠肝脏的30%。术后48小时，细胞核雌激素结合量比正常值升高了五倍。未观察到受体对雌激素的亲和力有改变。特异性研究表明，正常肝脏和再生肝脏中的雌激素受体相似，且对雌激素具有高度特异性。受体细胞分布的这些变化与肝脏中细胞核脱氧核糖核酸合成和有丝分裂指数的增加相一致。
