Finn W F, Fernandez-Repollet E, Goldfarb D, Iaina A, Eliahou H E
J Lab Clin Med. 1984 Feb;103(2):193-203.
The functional abnormalities associated with unilateral postischemic acute renal failure in the rat, as produced by 60 min of complete renal artery occlusion, are influenced by the presence or absence of the contralateral kidney. When the contralateral kidney is removed prior to the ischemia, reflow of blood to the postischemic kidney is more complete. To determine whether this maneuver influenced the ultimate severity of the injury and, if so, to investigate the mechanism by which this occurred, studies were performed conjointly at Tel-Hashomer, Israel, and Chapel Hill, N.C., in uninephrectomized (UNx) and sham-operated (Sh) rats. At Tel-Hashomer, V from UNx rats was nearly double that found in Sh rats at 1 to 3, 3 to 5, and 24 hr after ischemia. By 24 hr, CCr was substantially greater in UNx rats and was similar to that from the nonischemic kidney of Sh rats. Differences in tubular function were also noted. In UNx rats, FENa at 24 hr was significantly less and TCH2O was significantly greater than the corresponding values determined in Sh rats. At Chapel Hill, by 24 hr, RBF and PGCe in UNx rats were significantly greater than the corresponding values in Sh rats, although both were less than values obtained in nonischemic kidneys. In UNx rats, RT and RPG were considerably less than the corresponding values in Sh rats and similar to the values obtained in nonischemic kidneys. Microscopic examination at 24 hr revealed widespread tubular epithelial cell necrosis in Sh rats. In contrast, tubular epithelial cell structures were preserved in UNx rats. These data indicate that substantive differences exist in the response to temporary unilateral renal ischemia, which depend in part on the absence of the contralateral kidney and which result in less severe reduction in RBF and CCr and the preservation of tubular epithelial structure and function.
大鼠单侧缺血后急性肾衰竭相关的功能异常（由肾动脉完全闭塞60分钟所致）受对侧肾脏存在与否的影响。在缺血前切除对侧肾脏时，缺血后肾脏的血流再灌注更完全。为确定该操作是否影响损伤的最终严重程度，若有影响则探究其发生机制，在以色列的特尔哈绍梅尔和北卡罗来纳州的教堂山联合开展了研究，对象为单侧肾切除（UNx）大鼠和假手术（Sh）大鼠。在特尔哈绍梅尔，缺血后1至3小时、3至5小时及24小时，UNx大鼠的V值几乎是Sh大鼠的两倍。至24小时时，UNx大鼠的肌酐清除率（CCr）显著更高，且与Sh大鼠非缺血肾脏的CCr相似。还注意到肾小管功能存在差异。在UNx大鼠中，24小时时的尿钠排泄分数（FENa）显著更低，而自由水清除率（TCH2O）显著高于Sh大鼠的相应值。在教堂山，至24小时时，UNx大鼠的肾血流量（RBF）和肾小球滤过率（PGCe）显著高于Sh大鼠的相应值，尽管两者均低于非缺血肾脏的值。在UNx大鼠中，肾小管半径（RT）和肾小球毛细血管静水压（RPG）远低于Sh大鼠的相应值，且与非缺血肾脏的值相似。24小时时的显微镜检查显示，Sh大鼠存在广泛的肾小管上皮细胞坏死。相比之下，UNx大鼠的肾小管上皮细胞结构得以保留。这些数据表明，对暂时性单侧肾缺血的反应存在实质性差异，部分取决于对侧肾脏的缺失，这导致RBF和CCr的降低程度较轻，并保留了肾小管上皮结构和功能。
