Kyritsis A P, Tsokos M, Triche T J, Chader G J
Nature. 1984;307(5950):471-3. doi: 10.1038/307471a0.
The histogenesis of retinoblastoma, the most common intraocular neoplasm of childhood, remains controversial. Previous studies have attributed the origin of the tumour to neuronal, glial or primitive stem cells of retina. In the study described here we have used immunofluorescence to search for the presence of a neuronal marker, neurone-specific enolase (NSE) and a glial marker, glial fibrillary acidic protein (GFAP), in the cells of the human retinoblastoma line Y-79 (ref. 4), before and after successful differentiation into neuronal and glial-like cells. We found that all undifferentiated cells contain both NSE and GFAP, whereas the differentiating neuronal and glial-like cells gradually lose one marker and selectively express the marker that correlates with their morphology. Our results support the notion that retinoblastoma originates from a primitive bipotential (or multipotential) neuroectodermal cell.
视网膜母细胞瘤是儿童最常见的眼内肿瘤，其组织发生仍存在争议。以往的研究将该肿瘤的起源归因于视网膜的神经元、神经胶质或原始干细胞。在本文所述的研究中，我们利用免疫荧光技术，在人视网膜母细胞瘤细胞系Y-79（参考文献4）成功分化为神经元样细胞和神经胶质样细胞之前和之后，检测神经元标志物神经元特异性烯醇化酶（NSE）和神经胶质标志物胶质纤维酸性蛋白（GFAP）的存在情况。我们发现，所有未分化细胞均同时含有NSE和GFAP，而正在分化的神经元样细胞和神经胶质样细胞则逐渐失去一种标志物，并选择性地表达与其形态相关的标志物。我们的结果支持视网膜母细胞瘤起源于原始双潜能（或多潜能）神经外胚层细胞这一观点。
