Fishman M C, Schaffner A E
Am J Physiol. 1984 Jan;246(1 Pt 1):C106-13. doi: 10.1152/ajpcell.1984.246.1.C106.
Cells of the fetal carotid body have been obtained by enzymatic digestion and maintained in culture both as single cells and as clusters for up to 2 mo. The glomus cells in culture synthesize catecholamines and adenine nucleotides, as determined by histochemical methods, and contain characteristic dense-core vesicles. Their growth requirements are different from other cells of neural crest origin in that they do not depend on nerve growth factor (as do sympathetic neurons) or corticosteroids (as do SIF cells) for survival. Neither hypoxia nor hypercarbia affects survival or relative preponderance of glomus cells in culture. Tyrosine-free medium, which selects for cells containing tyrosine hydroxylase, eliminates most of the nonadrenergic cells, thereby providing a culture dramatically enhanced in glomus cells.
通过酶消化获得了胎儿颈动脉体的细胞，并将其作为单细胞和细胞簇在培养中维持长达2个月。通过组织化学方法测定，培养中的球细胞合成儿茶酚胺和腺嘌呤核苷酸，并含有特征性的致密核心小泡。它们的生长需求与其他神经嵴来源的细胞不同，因为它们的存活不依赖于神经生长因子（如交感神经元）或皮质类固醇（如SIF细胞）。缺氧和高碳酸血症均不影响培养中球细胞的存活或相对优势。不含酪氨酸的培养基可选择含有酪氨酸羟化酶的细胞，从而消除大多数非肾上腺素能细胞，从而提供一种球细胞显著增多的培养物。
