Fishman M C, Greene W L, Platika D
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1448-50. doi: 10.1073/pnas.82.5.1448.
Chemoreceptors for oxygen reside within the carotid body, but it is not known which cells actually sense hypoxia and by what mechanisms they transduce this information into afferent signals in the carotid sinus nerve. We have developed systems for the growth of glomus cells of the carotid body in dissociated cell culture. Here we demonstrate that, as in vivo, these cells contain the putative neurotransmitters dopamine, serotonin, and norepinephrine. Oxygen tension regulates the rate of dopamine secretion from the glomus cells. Similar to chemically stimulated catecholamine secretion from other adrenergic cells this hypoxia-stimulated release requires extracellular calcium. These results are compatible with the suggestion that the glomus cells of the carotid body are chemoreceptor cells and that they signal hypoxia by regulated secretion of dopamine.
氧化学感受器位于颈动脉体，但尚不清楚哪些细胞实际感知缺氧以及它们通过何种机制将该信息转化为颈动脉窦神经中的传入信号。我们已经开发出在解离细胞培养中培养颈动脉体球细胞的系统。在此我们证明，与体内情况一样，这些细胞含有假定的神经递质多巴胺、5-羟色胺和去甲肾上腺素。氧张力调节球细胞中多巴胺的分泌速率。与其他肾上腺素能细胞经化学刺激分泌儿茶酚胺类似，这种缺氧刺激的释放需要细胞外钙。这些结果与以下观点相符，即颈动脉体的球细胞是化学感受细胞，并且它们通过多巴胺的调节分泌来传递缺氧信号。
