Hayata S, Fujita M, Nakano Y, Kumagai M, Hakozaki M, Taguchi T
Gan To Kagaku Ryoho. 1984 Feb;11(2):270-8.
Human breast cancer (Br-13) serially transplanted in nude mice in ascitic form was used in this experiment. Released isozyme of human lactate dehydrogenase (LDH) was detected in the blood of the Br-13 tumor-bearing nude mice, and the serum levels of the isozyme rose associated with the increase of neoplastic cell number in ascitic fluid. After Br-13 bearing nude mice were treated with single drug or a combination of cyclophosphamide, adriamycin and 5-fluorouracil, serum LDH levels were monitored and compared with the change of ascitic cells. When effective treatment was performed, serum LDH-5 levels increased transitorily 1 or 2 days after drug administration, then decreased and became undetectable. These changes indicate a good correlation with the extent of the cell damage and the increase of life span of the mice. In conclusion, monitoring of human LDH isozyme in serum of nude mice was a useful marker for evaluating the efficacy of experimental chemotherapy in nude mice.
本实验使用以腹水形式在裸鼠体内连续传代移植的人乳腺癌（Br - 13）。在携带Br - 13肿瘤的裸鼠血液中检测到释放的人乳酸脱氢酶（LDH）同工酶，并且该同工酶的血清水平随着腹水中肿瘤细胞数量的增加而升高。在用环磷酰胺、阿霉素和5 - 氟尿嘧啶单一药物或联合药物治疗携带Br - 13的裸鼠后，监测血清LDH水平并与腹水细胞的变化进行比较。当进行有效治疗时，给药后1或2天血清LDH - 5水平短暂升高，然后下降并变得无法检测到。这些变化表明与细胞损伤程度和小鼠寿命延长有良好的相关性。总之，监测裸鼠血清中的人LDH同工酶是评估裸鼠实验性化疗疗效的有用标志物。
