腺苷甲硫氨酸和腺苷乙硫氨酸对甜菜碱-同型半胱氨酸甲基转移酶的失活作用。
Inactivation of betaine-homocysteine methyltransferase by adenosylmethionine and adenosylethionine.
作者信息
Finkelstein J D, Martin J J
出版信息
Biochem Biophys Res Commun. 1984 Jan 13;118(1):14-9. doi: 10.1016/0006-291x(84)91060-x.
PMID:6696751
Abstract
Preincubation of betaine-homocysteine methyltransferase, prepared from rat liver, with either S-adenosylmethionine or S-adenosylethionine results in a marked loss of enzyme activity. Gel filtration did not restore activity. However both S-adenosylhomocysteine and L-homocysteine, when added to the preincubation medium, inhibited the inactivation of betaine-homocysteine methyltransferase.
摘要
用大鼠肝脏制备的甜菜碱 - 同型半胱氨酸甲基转移酶，若与S - 腺苷甲硫氨酸或S - 腺苷乙硫氨酸进行预温育，会导致酶活性显著丧失。凝胶过滤不能恢复活性。然而，当将S - 腺苷同型半胱氨酸和L - 同型半胱氨酸添加到预温育培养基中时，它们会抑制甜菜碱 - 同型半胱氨酸甲基转移酶的失活。
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