Dykes P J, Williams D L, Marks R
Br J Dermatol. 1984 Mar;110(3):283-92. doi: 10.1111/j.1365-2133.1984.tb04633.x.
Mechanical stimulation of the skin surface in the presence of non-ionic detergent (Triton X-100)-containing buffers has been used to prepare isolated corneocytes and non-ionic detergent-soluble (NIDS) material from human stratum corneum. Analysis of the major lipid classes present in the corneocytes and NIDS material has been performed by thin layer chromatography with flame ionization detection and quantitation based on the introduction of an internal standard. Results indicate that the NIDS lipid represents a disproportionately large amount of the total stratum corneum lipid and that it differs in composition from the corneocyte lipid, there being a greater proportion of the cholesteryl ester fraction in the corneocytes. Both these findings support the 'brick wall' model of the stratum corneum and provide further evidence that the NIDS material is intercellular in nature.
在含有非离子洗涤剂（Triton X-100）的缓冲液中对皮肤表面进行机械刺激，已被用于从人角质层制备分离的角质形成细胞和非离子洗涤剂可溶性（NIDS）物质。通过带有火焰离子化检测的薄层色谱法并基于内标的引入进行定量，对角质形成细胞和NIDS物质中存在的主要脂质类别进行了分析。结果表明，NIDS脂质在角质层总脂质中占比过大，并且其组成与角质形成细胞脂质不同，角质形成细胞中胆固醇酯部分的比例更高。这两个发现都支持角质层的“砖墙”模型，并进一步证明NIDS物质本质上是细胞间的。
