Kähäri V M, Vuorio T, Näntö-Salonen K, Vuorio E
Biochim Biophys Acta. 1984 Feb 24;781(1-2):183-6. doi: 10.1016/0167-4781(84)90136-2.
Pro alpha 1(I)collagen mRNA levels in fibroblasts cultured from affected and non-affected skin areas of two scleroderma patients were measured by hybridization of RNA blots with a specific cDNA clone. Collagen synthesis was estimated with an inhibition ELISA for type I collagen and with densitometric scans of fluorograms of [3H]proline-labelled medium proteins. Affected scleroderma fibroblasts exhibited 2-7-fold higher levels of pro alpha 1(I)collagen mRNAs to account for the increased synthesis of collagen by the same cells. This suggests that the control of collagen synthesis in scleroderma is altered at transcriptional level.
通过用特定的cDNA克隆对RNA印迹进行杂交，测定了两名硬皮病患者受影响和未受影响皮肤区域培养的成纤维细胞中前α1（I）型胶原蛋白mRNA的水平。用I型胶原蛋白抑制ELISA法以及对[3H]脯氨酸标记的培养基蛋白荧光图进行光密度扫描来估计胶原蛋白的合成。硬皮病成纤维细胞中前α1（I）型胶原蛋白mRNA水平高出2至7倍，以解释同一细胞中胶原蛋白合成的增加。这表明硬皮病中胶原蛋白合成的控制在转录水平上发生了改变。
