Cavalieri R L, Kowalczyk A A, Davenport L, Chin C, Brand L
Biochim Biophys Acta. 1984 Feb 9;792(2):158-63. doi: 10.1016/0005-2760(84)90217-0.
The fluorescence emission anisotropy of 1,6-diphenyl-1,3,5-hexatriene in human amniotic fluid has been studied using nanosecond time-resolved emission techniques. These studies demonstrate that the previously reported decrease in the steady state emission anisotropy, [r], with gestational age is due to a change in the rate of rotational motion of the probe. The emission anisotropy decays to a limiting value (r infinity) greater than zero, suggesting a hindered rotation of the probe, and this is independent of gestational age. The decay function for the emission anisotropy of amniotic fluids from 17, 29, 40 and 41 weeks in gestational age can be best expressed as a single exponential plus a constant term, with rotational correlation times varying from 17 ns to 2.2 ns, respectively. The zero time emission anisotropy remains approx. 0.30 for both early and late gestational times.
利用纳秒时间分辨发射技术研究了人羊水中1,6 - 二苯基 - 1,3,5 - 己三烯的荧光发射各向异性。这些研究表明，先前报道的稳态发射各向异性[r]随胎龄的降低是由于探针旋转运动速率的变化。发射各向异性衰减到大于零的极限值（r∞），表明探针旋转受阻，且这与胎龄无关。孕龄为17、29、40和41周的羊水发射各向异性的衰减函数可以最好地表示为单指数加常数项，旋转相关时间分别从17纳秒到2.2纳秒不等。早期和晚期孕龄的零时间发射各向异性均约为0.30。
