通过多频相位调制荧光测定法获得的溶剂和脂质双层中二苯基己三烯和苝的时间分辨荧光各向异性。
Time-resolved fluorescence anisotropies of diphenylhexatriene and perylene in solvents and lipid bilayers obtained from multifrequency phase-modulation fluorometry.
作者信息
Lakowicz J R, Cherek H, Maliwal B P
出版信息
Biochemistry. 1985 Jan 15;24(2):376-83. doi: 10.1021/bi00323a021.
Abstract
Time-resolved decays of fluorescence anisotropy were obtained from frequency-domain measurements of the phase angle difference between the parallel and perpendicular components of the polarized emission and the ratio of the modulated amplitudes. These data were measured at modulation frequencies ranging from 1 to 200 MHz. To demonstrate the general applicability of this method, we describe the resolution of both simple and complex decays of anisotropy. In particular, we resolved single, double, and triple exponential decays of anisotropy and the hindered rotational motions of fluorophores within lipid bilayers. The ease and rapidity with which these results were obtained indicate that frequency-domain measurements are both practical and reliable for the determination of complex decays of anisotropy.
摘要
通过对偏振发射的平行和垂直分量之间的相位角差以及调制幅度比进行频域测量，获得了荧光各向异性的时间分辨衰减。这些数据是在1至200 MHz的调制频率下测量的。为了证明该方法的普遍适用性，我们描述了简单和复杂各向异性衰减的分辨率。特别是，我们解析了各向异性的单指数、双指数和三指数衰减以及脂质双层中荧光团的受阻旋转运动。获得这些结果的简便性和快速性表明，频域测量对于确定复杂的各向异性衰减既实用又可靠。
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