Rådmark O, Shimizu T, Fitzpatrick F, Samuelsson B
Biochim Biophys Acta. 1984 Mar 7;792(3):324-9. doi: 10.1016/0005-2760(84)90200-5.
Incubation of various hemoproteins with 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid or 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid resulted in formation of epimeric 5(S),12-dihydroxy-6,8,10,14 -eicosatetraenoic acids and epimeric 8,15(S)-dihydroxy-5,9,11,13 -eicosatetraenoic acids, respectively. These dihydroxy acids were earlier recognized as nonenzymatic hydrolysis products of 5(S),6-oxido-7,9,11,14-eicosatetraenoic acid (leukotriene A4) and 14,15(S)-oxido-5,8,10,12-eicosatetraenoic acid (14,15-leukotriene A4). These allylic epoxides could be isolated as such from the hemoprotein incubations, and most probably they are intermediates in formation of the dihydroxy acids.
将各种血红素蛋白与5-氢过氧-6,8,11,14-二十碳四烯酸或15-氢过氧-5,8,11,13-二十碳四烯酸一起孵育，分别导致形成差向异构的5(S),12-二羟基-6,8,10,14-二十碳四烯酸和差向异构的8,15(S)-二羟基-5,9,11,13-二十碳四烯酸。这些二羟基酸先前被认为是5(S),6-环氧-7,9,11,14-二十碳四烯酸（白三烯A4）和14,15(S)-环氧-5,8,10,12-二十碳四烯酸（14,15-白三烯A4）的非酶促水解产物。这些烯丙基环氧化物可以从血红素蛋白孵育物中直接分离得到，并且它们很可能是二羟基酸形成过程中的中间体。
