Skvortsov V T, Gurvich A E
Biull Eksp Biol Med. 1984 Feb;97(2):179-80.
A highly capacious immunosorbent for antigen demonstration has been synthesized. Use has been made of oriented immobilization of antibody Fab'-fragments on porous cellulose balls at the expense of the reaction of disulfide exchange. Up to 100 mg of Fab' was fixed on the sorbent, with the capacity reaching 200 mg antigen (rabbit IgG) per g of dry weight sorbent. The molar ratio Fab'/IgG was equal to 0.8-0.9, i.e. approximated the theoretical limit: one molecule of antigen per one active center antibody.
已合成一种用于抗原展示的高容量免疫吸附剂。利用二硫键交换反应将抗体Fab'片段定向固定在多孔纤维素球上。每克干重吸附剂上固定了高达100毫克的Fab'，吸附容量达到200毫克抗原（兔IgG）。Fab'/IgG的摩尔比为0.8 - 0.9，即接近理论极限：一个活性中心抗体对应一个抗原分子。
