Coulomb H M, Gu Z W, Loireau M P, Chouroulinkov I
Cancer Lett. 1984 Feb;22(1):77-82. doi: 10.1016/0304-3835(84)90046-6.
The role of serum concentration on the cytotoxicity and on the sister chromatid exchange (SCE)-induction by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and by a diesel particulate extract (DPE), a complex mixture, has been carried out on V79 cells. An increase of the serum concentration in the medium decreases the toxicity of these chemicals, and especially when they are dispersed first in serum. Although no influence of serum concentration on the number of spontaneous SCEs occurring in control cells has been observed, the increase of serum concentration leads to a decrease in SCE's induction in treated cells. Our results show that serum can protect cells from the cytotoxic and mutagenic action of MNNG and diesel extract.
在V79细胞上研究了血清浓度对N-甲基-N'-硝基-N-亚硝基胍（MNNG）和柴油颗粒提取物（DPE，一种复杂混合物）的细胞毒性及姐妹染色单体交换（SCE）诱导作用的影响。培养基中血清浓度的增加会降低这些化学物质的毒性，尤其是当它们先在血清中分散时。虽然未观察到血清浓度对对照细胞中自发SCE数量有影响，但血清浓度的增加会导致处理细胞中SCE诱导的减少。我们的结果表明，血清可以保护细胞免受MNNG和柴油提取物的细胞毒性和诱变作用。
