Sakamoto S, Iwama T, Tsukada K, Utsunomiya J, Kawasaki T, Okamoto R
Carcinogenesis. 1984 Feb;5(2):183-5. doi: 10.1093/carcin/5.2.183.
Thymidine kinase (TK), the salvage pathway enzyme, and its isozymes were examined in normal mucosa and carcinoma of the colon in 13 patients. In colon tumor TK activity increased to 328% of activity of normal colon. The colon TK isozymes were separated from the normal mucosa and carcinoma by DEAE-cellulose column chromatography. The TK isozyme eluted from the column by the elution buffer alone without NaCl was markedly higher (12-fold) in activity in carcinoma than in normal mucosa of the colon and is not affected by dCTP. The TK isozyme unaffected by dCTP has also been reported to be clearly involved in DNA synthesis. This isozyme, whose molecular weight is 100 000 by means of high performance liquid chromatography, is thought to be involved in DNA replication. The results indicate the applicability of the molecular correlation concept to carcinogenesis of colon.
对13例患者的正常结肠黏膜和结肠癌组织中的补救途径酶——胸苷激酶（TK）及其同工酶进行了检测。在结肠肿瘤中，TK活性增加至正常结肠活性的328%。通过DEAE - 纤维素柱色谱法从正常黏膜和癌组织中分离出结肠TK同工酶。仅用洗脱缓冲液而不加NaCl从柱上洗脱下来的TK同工酶，其在癌组织中的活性比结肠正常黏膜中的活性明显更高（12倍），且不受dCTP的影响。据报道，不受dCTP影响的TK同工酶也明显参与DNA合成。通过高效液相色谱法测得该同工酶的分子量为100000，被认为参与DNA复制。结果表明分子关联概念在结肠癌发生过程中的适用性。
