Sakamoto S, Iwama T, Ebuchi M, Tsukada K, Sagara T, Kawasaki T, Murakami S, Kasahara N, Kudo H, Okamoto R
Br J Surg. 1986 Apr;73(4):272-3. doi: 10.1002/bjs.1800730409.
Thymidine kinase, the enzyme in the pyrimidine salvage pathway, and its isozymes were examined in 10 specimens of normal mammary gland, 10 fibroadenomas and 11 adenocarcinomas in human breasts. The average thymidine kinase activities in fibroadenomas and adenocarcinomas were about 3 and 8 times that in normal mammary gland. The mammary thymidine kinase isozymes were separated into two types by diethylaminoethyl (DEAE) cellulose column chromatography. The activity of the thymidine kinase isozyme eluted with 0.1 M sodium chloride in buffer was twofold higher in fibroadenomas and fourfold higher in adenocarcinomas than that in normal tissue. In adenocarcinomas, but not fibroadenomas, the activity of the other isozyme eluted with buffer alone was increased to 22-fold that in normal tissues. As the activity of the latter isozyme was not affected by deoxycytidine triphosphate, it may be involved closely in DNA replication.
在人乳腺的10份正常组织、10份纤维腺瘤和11份腺癌标本中检测了嘧啶补救途径中的胸苷激酶及其同工酶。纤维腺瘤和腺癌中的胸苷激酶平均活性分别约为正常乳腺组织的3倍和8倍。乳腺胸苷激酶同工酶通过二乙氨基乙基（DEAE）纤维素柱色谱法分为两种类型。在缓冲液中用0.1M氯化钠洗脱的胸苷激酶同工酶活性在纤维腺瘤中比正常组织高两倍，在腺癌中高四倍。在腺癌中，而非纤维腺瘤中，仅用缓冲液洗脱的另一种同工酶活性增加至正常组织的22倍。由于后一种同工酶的活性不受三磷酸脱氧胞苷的影响，它可能与DNA复制密切相关。
