Tracy R P, Young D S
Clin Chem. 1984 Mar;30(3):462-5.
We describe a microcomputer-based densitometer that includes a television camera for the analysis of one- and two-dimensional electrophoresis gels. The hardware configuration is a modification of that described by Mariash et al. (Anal Biochem 212: 388-394, 1982). The video signal is digitized by a commercially available interface, yielding 1 of 64 greyscale values for each pixel examined. The operator controls the region being analyzed through the use of a joystick and video monitor. We have optimized the linear response range (0-1.5 A) and have written a relatively simple control program ("GELSCAN 5.0"). For analyses of one-dimensional gels, the required operator decisions include the start and finish points of the gel, the width of the scan (analogous to slit size), and the level of background correction. The digital information may be stored on disk, displayed by one of several graphic routines, and analyzed for total scan and individual peak absorbance values. Similar analyses may be done with two-dimensional gel spots.
我们描述了一种基于微型计算机的密度计，它包括一个用于分析一维和二维电泳凝胶的电视摄像机。硬件配置是对Mariash等人（《分析生物化学》212:388 - 394,1982）所描述的配置的一种改进。视频信号通过一个商用接口进行数字化处理，对于每个被检测的像素产生64个灰度值中的1个。操作人员通过使用操纵杆和视频监视器来控制被分析的区域。我们已经优化了线性响应范围（0 - 1.5 A），并编写了一个相对简单的控制程序（“GELSCAN 5.0”）。对于一维凝胶的分析，操作人员需要做出的决策包括凝胶的起始点和终点、扫描宽度（类似于狭缝大小）以及背景校正水平。数字信息可以存储在磁盘上，通过几种图形程序之一进行显示，并分析总扫描值和各个峰吸光度值。二维凝胶斑点也可以进行类似的分析。
