矿物质对葡萄球菌肠毒素B产生的影响。
Effect of minerals on staphylococcal enterotoxin B production.
作者信息
Keller G M, Hanson R S, Bergdoll M S
出版信息
Infect Immun. 1978 Apr;20(1):158-60. doi: 10.1128/iai.20.1.158-160.1978.
Abstract
Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting growth and enterotoxin production. The optimum level of potassium was between 15 and 45 mM. Ammonium salts were not essential in the amino acid medium. Sufficient quantities of trace metals were already present. Revising the salt mixture according to the information obtained resulted in a doubling of the enterotoxin B production.
摘要
在仅含有氨基酸（2.08%）、盐类和维生素的培养基中，将镁离子浓度从0.4 mM提高到1.5 mM，可使金黄色葡萄球菌S-6的肠毒素B产量增加约80%。培养基中磷酸盐的水平降至原来的十分之一（至2.87 mM），而不会对生长和肠毒素产生产生不利影响。钾的最佳水平在15至45 mM之间。在氨基酸培养基中铵盐并非必需。已经存在足够数量的痕量金属。根据所获得的信息修改盐混合物，可使肠毒素B产量翻倍。
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