Miller R D, Fung D Y
Appl Microbiol. 1973 May;25(5):800-6. doi: 10.1128/am.25.5.800-806.1973.
A minimal chemically defined medium has been developed for growth (approximately 25 Klett units) and production of detectable enterotoxin B (approximately 5-6 mug/ml) by Staphylococcus aureus S-6. This medium contains monosodium glutamate as a source of carbon, nitrogen, and energy, three additional amino acids (arginine, cystine, and phenylalanine), six inorganic salts, and four vitamins. Increasing the concentrations of several amino acids in a series of defined media gave no increase in enterotoxin production. Apparently the limiting factor for growth and enterotoxin production in these media is the biosynthesis of one or more missing amino acids, rather than the concentration of the amino acids present in the media. An additional requirement for proline and valine was observed when glucose was added as the primary source of energy. When compared to complex media, our results indicated that the inhibitory effect of glucose on enterotoxin synthesis in defined media was less evident or totally absent.
已开发出一种化学成分确定的基本培养基，用于金黄色葡萄球菌S-6的生长（约25个光密度单位）和可检测肠毒素B的产生（约5-6微克/毫升）。该培养基含有谷氨酸钠作为碳、氮和能量的来源，另外三种氨基酸（精氨酸、胱氨酸和苯丙氨酸），六种无机盐和四种维生素。在一系列化学成分确定的培养基中增加几种氨基酸的浓度，肠毒素产量并未增加。显然，这些培养基中生长和肠毒素产生的限制因素是一种或多种缺失氨基酸的生物合成，而不是培养基中存在的氨基酸浓度。当添加葡萄糖作为主要能量来源时，观察到对脯氨酸和缬氨酸有额外需求。与复杂培养基相比，我们的结果表明，葡萄糖对化学成分确定的培养基中肠毒素合成的抑制作用不太明显或完全不存在。
