Livermore B P, Bey R F, Johnson R C
Infect Immun. 1978 Apr;20(1):215-20. doi: 10.1128/iai.20.1.215-220.1978.
The synthesis of complex lipids by Borrelia hermsi while growing in Kelly medium was investigated by labeling cultures with D-[14C]glucose or [14C]palmitic acid. Labeled glucose was incorporated into phosphatidyl choline, phosphatidyl glycerol, monogalactosyl diglyceride, cholesterol glucoside, and acylated cholesteryl glucoside. The fatty acid composition reflected that of the medium suggesting that this spirochete directly incorporates acyl chains unaltered from its external milieu. Furthermore, the distribution of labeled carbon between acyl groups (lipid-soluble) and water-soluble moieties indicates that there is no metabolic exchange between these two pools. The relationship of B. hermsi to other spirochetes is discussed in terms of lipid metabolism as a generic characteristic.
通过用D-[14C]葡萄糖或[14C]棕榈酸标记培养物，研究了赫氏疏螺旋体在凯利培养基中生长时复合脂质的合成。标记的葡萄糖被整合到磷脂酰胆碱、磷脂酰甘油、单半乳糖基甘油二酯、胆固醇葡萄糖苷和酰化胆固醇葡萄糖苷中。脂肪酸组成反映了培养基的脂肪酸组成，这表明这种螺旋体直接从其外部环境中摄取未改变的酰基链。此外，标记碳在酰基（脂溶性）和水溶性部分之间的分布表明，这两个池之间没有代谢交换。从脂质代谢作为一个共同特征的角度讨论了赫氏疏螺旋体与其他螺旋体的关系。
